Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa
Identifieur interne : 00B163 ( Main/Exploration ); précédent : 00B162; suivant : 00B164Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa
Auteurs : Mgaly De Chial [Belgique] ; Bart Ghysels [Belgique] ; Scott A. Beatson [Royaume-Uni] ; Valérie Geoffroy [France] ; Jean Marie Meyer [France] ; Theresa Pattery [Belgique] ; Christine Baysse [Belgique] ; Patrice Chablain [Belgique] ; Yasmin N. Parsons [Royaume-Uni] ; Craig Winstanley [Royaume-Uni] ; Stuart J. Cordwell [Australie] ; Pierre Cornelis [Belgique]Source :
- Microbiology : (Reading) [ 1350-0872 ] ; 2003.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
Pseudomonas aeruginosa produces, under conditions of iron limitation, a high-affinity siderophore, pyoverdine (PVD), which is recognized at the level of the outer membrane by a specific TonB-dependent receptor, FpvA. So far, for P. aeruginosa, three different PVDs, differing in their peptide chain, have been described (types I-III), but only the FpvA receptor for type I is known. Two PVD-producing P. aeruginosa strains, one type II and one type III, were mutagenized by a mini-TnphoA3 transposon. In each case, one mutant unable to grow in the presence of the strong iron chelator ethylenediaminedihydroxyphenylacetic acid (EDDHA) and the cognate PVD was selected. The first mutant, which had an insertion in the pvdE gene, upstream of fpvA, was unable to take up type II PVD and showed resistance to pyocin S3, which is known to use type II FpvA as receptor. The second mutant was unable to take up type III PVD and had the transposon insertion in fpvA. Cosmid libraries of the respective type II and type III PVD wild-type strains were constructed and screened for clones restoring the capacity to grow in the presence of PVD. From the respective complementing genomic fragments, type II and type III fpvA sequences were determined. When in trans, type II and type III fpvA restored PVD production, uptake, growth in the presence of EDDHA and, in the case of type II fpvA, pyocin S3 sensitivity. Complementation of fpvA mutants obtained by allelic exchange was achieved by the presence of cognate fpvA in trans. All three receptors posses an N-terminal extension of about 70 amino acids, similar to FecA of Escherichia coli, but only FpvAl has a TAT export sequence at its N-terminal end.
Affiliations:
- Australie, Belgique, France, Royaume-Uni
- Alsace (région administrative), Angleterre, Grand Est, Oxfordshire, Vienne (Autriche)
- Oxford, Strasbourg, Vienne (Autriche)
- Université Strasbourg 1, Université d'Oxford
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Cordwell</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Australian Proteome Analysis Facility</s1><s2>Sydney, 2109</s2><s3>AUS</s3><sZ>11 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Australian Proteome Analysis Facility</wicri:noRegion></affiliation></author><author><name sortKey="Cornelis, Pierre" sort="Cornelis, Pierre" uniqKey="Cornelis P" first="Pierre" last="Cornelis">Pierre Cornelis</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Flanders Interuniversity Institute of Biotechnology (VIB6), Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E, room 6.6, Pleinlaan 2</s1><s2>1050 Brussels</s2><s3>BEL</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Belgique</country><placeName><region type="land" nuts="2">Vienne (Autriche)</region><settlement type="city">Vienne (Autriche)</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">04-0504392</idno><date when="2003">2003</date><idno type="stanalyst">PASCAL 04-0504392 INIST</idno><idno type="RBID">Pascal:04-0504392</idno><idno type="wicri:Area/PascalFrancis/Corpus">004C92</idno><idno type="wicri:Area/PascalFrancis/Curation">001422</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">004F43</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">004F43</idno><idno type="wicri:doubleKey">1350-0872:2003:De Chial M:identification:of:type</idno><idno type="wicri:Area/Main/Merge">00BF28</idno><idno type="wicri:Area/Main/Curation">00B163</idno><idno type="wicri:Area/Main/Exploration">00B163</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa</title><author><name sortKey="De Chial, Mgaly" sort="De Chial, Mgaly" uniqKey="De Chial M" first="Mgaly" last="De Chial">Mgaly De Chial</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Flanders Interuniversity Institute of Biotechnology (VIB6), Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E, room 6.6, Pleinlaan 2</s1><s2>1050 Brussels</s2><s3>BEL</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Belgique</country><placeName><region type="land" nuts="2">Vienne (Autriche)</region><settlement type="city">Vienne (Autriche)</settlement></placeName></affiliation></author><author><name sortKey="Ghysels, Bart" sort="Ghysels, Bart" uniqKey="Ghysels B" first="Bart" last="Ghysels">Bart Ghysels</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Flanders Interuniversity Institute of Biotechnology (VIB6), Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E, room 6.6, Pleinlaan 2</s1><s2>1050 Brussels</s2><s3>BEL</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Belgique</country><placeName><region type="land" nuts="2">Vienne (Autriche)</region><settlement type="city">Vienne (Autriche)</settlement></placeName></affiliation></author><author><name sortKey="Beatson, Scott A" sort="Beatson, Scott A" uniqKey="Beatson S" first="Scott A." last="Beatson">Scott A. Beatson</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>MRC Functional Genetics Unit, Department of Human Anatomy and Genetics, University of Oxford, South Parks Road</s1><s2>Oxford OX1 3QX</s2><s3>GBR</s3><sZ>3 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Oxford OX1 3QX</wicri:noRegion><orgName type="university">Université d'Oxford</orgName><placeName><settlement type="city">Oxford</settlement><region type="nation">Angleterre</region><region type="région" nuts="1">Oxfordshire</region></placeName></affiliation></author><author><name sortKey="Geoffroy, Valerie" sort="Geoffroy, Valerie" uniqKey="Geoffroy V" first="Valérie" last="Geoffroy">Valérie Geoffroy</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Laboratoire de Microbiologie et de Génétique, Université Louis Pasteur, UPRES-A 7010</s1><s2>67000 Strasbourg</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>67000 Strasbourg</wicri:noRegion><placeName><settlement type="city">Strasbourg</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName><orgName type="university">Université Strasbourg 1</orgName></affiliation></author><author><name sortKey="Meyer, Jean Marie" sort="Meyer, Jean Marie" uniqKey="Meyer J" first="Jean Marie" last="Meyer">Jean Marie Meyer</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Laboratoire de Microbiologie et de Génétique, Université Louis Pasteur, UPRES-A 7010</s1><s2>67000 Strasbourg</s2><s3>FRA</s3><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>67000 Strasbourg</wicri:noRegion><placeName><settlement type="city">Strasbourg</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName><orgName type="university">Université Strasbourg 1</orgName></affiliation></author><author><name sortKey="Pattery, Theresa" sort="Pattery, Theresa" uniqKey="Pattery T" first="Theresa" last="Pattery">Theresa Pattery</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Flanders Interuniversity Institute of Biotechnology (VIB6), Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E, room 6.6, Pleinlaan 2</s1><s2>1050 Brussels</s2><s3>BEL</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Belgique</country><placeName><region type="land" nuts="2">Vienne (Autriche)</region><settlement type="city">Vienne (Autriche)</settlement></placeName></affiliation></author><author><name sortKey="Baysse, Christine" sort="Baysse, Christine" uniqKey="Baysse C" first="Christine" last="Baysse">Christine Baysse</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Flanders Interuniversity Institute of Biotechnology (VIB6), Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E, room 6.6, Pleinlaan 2</s1><s2>1050 Brussels</s2><s3>BEL</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Belgique</country><placeName><region type="land" nuts="2">Vienne (Autriche)</region><settlement type="city">Vienne (Autriche)</settlement></placeName></affiliation></author><author><name sortKey="Chablain, Patrice" sort="Chablain, Patrice" uniqKey="Chablain P" first="Patrice" last="Chablain">Patrice Chablain</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Flanders Interuniversity Institute of Biotechnology (VIB6), Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E, room 6.6, Pleinlaan 2</s1><s2>1050 Brussels</s2><s3>BEL</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Belgique</country><placeName><region type="land" nuts="2">Vienne (Autriche)</region><settlement type="city">Vienne (Autriche)</settlement></placeName></affiliation></author><author><name sortKey="Parsons, Yasmin N" sort="Parsons, Yasmin N" uniqKey="Parsons Y" first="Yasmin N." last="Parsons">Yasmin N. Parsons</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building</s1><s2>Liverpool L69 3GA</s2><s3>GBR</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Liverpool L69 3GA</wicri:noRegion></affiliation></author><author><name sortKey="Winstanley, Craig" sort="Winstanley, Craig" uniqKey="Winstanley C" first="Craig" last="Winstanley">Craig Winstanley</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Medical Microbiology and Genitourinary Medicine, University of Liverpool, Duncan Building</s1><s2>Liverpool L69 3GA</s2><s3>GBR</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Liverpool L69 3GA</wicri:noRegion></affiliation></author><author><name sortKey="Cordwell, Stuart J" sort="Cordwell, Stuart J" uniqKey="Cordwell S" first="Stuart J." last="Cordwell">Stuart J. Cordwell</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Australian Proteome Analysis Facility</s1><s2>Sydney, 2109</s2><s3>AUS</s3><sZ>11 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Australian Proteome Analysis Facility</wicri:noRegion></affiliation></author><author><name sortKey="Cornelis, Pierre" sort="Cornelis, Pierre" uniqKey="Cornelis P" first="Pierre" last="Cornelis">Pierre Cornelis</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Flanders Interuniversity Institute of Biotechnology (VIB6), Laboratory of Microbial Interactions, Vrije Universiteit Brussel, Building E, room 6.6, Pleinlaan 2</s1><s2>1050 Brussels</s2><s3>BEL</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Belgique</country><placeName><region type="land" nuts="2">Vienne (Autriche)</region><settlement type="city">Vienne (Autriche)</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Microbiology : (Reading)</title><title level="j" type="abbreviated">Microbiology : (Read.)</title><idno type="ISSN">1350-0872</idno><imprint><date when="2003">2003</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Microbiology : (Reading)</title><title level="j" type="abbreviated">Microbiology : (Read.)</title><idno type="ISSN">1350-0872</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Identification</term><term>Pseudomonas aeruginosa</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Pseudomonas aeruginosa</term><term>Identification</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pseudomonas aeruginosa produces, under conditions of iron limitation, a high-affinity siderophore, pyoverdine (PVD), which is recognized at the level of the outer membrane by a specific TonB-dependent receptor, FpvA. So far, for P. aeruginosa, three different PVDs, differing in their peptide chain, have been described (types I-III), but only the FpvA receptor for type I is known. Two PVD-producing P. aeruginosa strains, one type II and one type III, were mutagenized by a mini-TnphoA3 transposon. In each case, one mutant unable to grow in the presence of the strong iron chelator ethylenediaminedihydroxyphenylacetic acid (EDDHA) and the cognate PVD was selected. The first mutant, which had an insertion in the pvdE gene, upstream of fpvA, was unable to take up type II PVD and showed resistance to pyocin S3, which is known to use type II FpvA as receptor. The second mutant was unable to take up type III PVD and had the transposon insertion in fpvA. Cosmid libraries of the respective type II and type III PVD wild-type strains were constructed and screened for clones restoring the capacity to grow in the presence of PVD. From the respective complementing genomic fragments, type II and type III fpvA sequences were determined. When in trans, type II and type III fpvA restored PVD production, uptake, growth in the presence of EDDHA and, in the case of type II fpvA, pyocin S3 sensitivity. Complementation of fpvA mutants obtained by allelic exchange was achieved by the presence of cognate fpvA in trans. All three receptors posses an N-terminal extension of about 70 amino acids, similar to FecA of Escherichia coli, but only FpvAl has a TAT export sequence at its N-terminal end.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Belgique</li><li>France</li><li>Royaume-Uni</li></country><region><li>Alsace (région administrative)</li><li>Angleterre</li><li>Grand Est</li><li>Oxfordshire</li><li>Vienne (Autriche)</li></region><settlement><li>Oxford</li><li>Strasbourg</li><li>Vienne (Autriche)</li></settlement><orgName><li>Université Strasbourg 1</li><li>Université d'Oxford</li></orgName></list><tree><country name="Belgique"><region name="Vienne (Autriche)"><name sortKey="De Chial, Mgaly" sort="De Chial, Mgaly" uniqKey="De Chial M" first="Mgaly" last="De Chial">Mgaly De Chial</name></region><name sortKey="Baysse, Christine" sort="Baysse, Christine" uniqKey="Baysse C" first="Christine" last="Baysse">Christine Baysse</name><name sortKey="Chablain, Patrice" sort="Chablain, Patrice" uniqKey="Chablain P" first="Patrice" last="Chablain">Patrice Chablain</name><name sortKey="Cornelis, Pierre" sort="Cornelis, Pierre" uniqKey="Cornelis P" first="Pierre" last="Cornelis">Pierre Cornelis</name><name sortKey="Ghysels, Bart" sort="Ghysels, Bart" uniqKey="Ghysels B" first="Bart" last="Ghysels">Bart Ghysels</name><name sortKey="Pattery, Theresa" sort="Pattery, Theresa" uniqKey="Pattery T" first="Theresa" last="Pattery">Theresa Pattery</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Beatson, Scott A" sort="Beatson, Scott A" uniqKey="Beatson S" first="Scott A." last="Beatson">Scott A. Beatson</name></region><name sortKey="Parsons, Yasmin N" sort="Parsons, Yasmin N" uniqKey="Parsons Y" first="Yasmin N." last="Parsons">Yasmin N. Parsons</name><name sortKey="Winstanley, Craig" sort="Winstanley, Craig" uniqKey="Winstanley C" first="Craig" last="Winstanley">Craig Winstanley</name></country><country name="France"><region name="Grand Est"><name sortKey="Geoffroy, Valerie" sort="Geoffroy, Valerie" uniqKey="Geoffroy V" first="Valérie" last="Geoffroy">Valérie Geoffroy</name></region><name sortKey="Meyer, Jean Marie" sort="Meyer, Jean Marie" uniqKey="Meyer J" first="Jean Marie" last="Meyer">Jean Marie Meyer</name></country><country name="Australie"><noRegion><name sortKey="Cordwell, Stuart J" sort="Cordwell, Stuart J" uniqKey="Cordwell S" first="Stuart J." last="Cordwell">Stuart J. Cordwell</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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